![]() J Biol Chem 284(15):9637–9641īogdanov M, Heacock PN, Dowhan W (2010a) Study of polytopic membrane protein topological organization as a function of membrane lipid composition. J Cell Biol 182(5):925–935īogdanov M, Xie J, Dowhan W (2009) Lipid-protein interactions drive membrane protein topogenesis in accordance with the positive inside rule. Methods 36(2):148–171īogdanov M, Xie J, Heacock P, Dowhan W (2008) To flip or not to flip: lipid-protein charge interactions are a determinant of final membrane protein topology. EMBO J 21(9):2107–2116īogdanov M, Zhang W, Xie J, Dowhan W (2005) Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAM™): application to lipid-specific membrane protein topogenesis. J Biol Chem 287:37939–37948īogdanov M, Heacock PN, Dowhan W (2002) A polytopic membrane protein displays a reversible topology dependent on membrane lipid composition. In: Bacterial protein secretion systems: methods and protocols, pp 105–128īogdanov M, Dowhan W (2012) Lipid-dependent generation of a dual topology for a membrane protein. ![]() Biochem Soc Trans 41(1):35–42īogdanov M (2017) Mapping of membrane protein topology by substituted cysteine accessibility method (SCAM™). FEBS Lett 582(21–22):3179–3184īochud A, Ramachandra N, Conzelmann A (2013) Adaptation of low-resolution methods for the study of yeast microsomal polytopic membrane proteins: a methodological review. This process is experimental and the keywords may be updated as the learning algorithm improves.Īwe K, Lambert C, Prange R (2008) Mammalian BiP controls posttranslational ER translocation of the hepatitis B virus large envelope protein. These keywords were added by machine and not by the authors. Förster Resonance Energy Transfer (FRET).Such methods have the advantage of probing the dynamics of biological membrane organization, membrane protein structure, and lipid-protein interactions both in vitro and in vivo. ![]() Novel approaches and concepts have been developed to analyze dynamic lipid-protein interactions and mechanisms of membrane protein folding and topogenesis. Therefore, a set of unique in vivo and in vitro approaches should be used to establish a detailed mechanistic understanding for how lipid-protein interactions govern dynamic membrane protein structure and function. A structural approach for dynamic membrane protein organization is not achievable by X-ray crystallography. Phosphorylation of an extramembrane domain, which alters its charge nature, could also induce post-insertional topological changes. Membrane proteins can be also engineered to flip after assembly if a strong topological retention signal is introduced at the very end of the polypeptide and then removed post-insertionally. Although the lipid bilayer is widely considered as a non-flipping zone for most proteins, some integral membrane proteins possess the capacity to reversibly reorient themselves during or after insertion if membrane phospholipid composition is changed, the membrane is depolarized or components of the translocon interact with each other during ATP-driven protein substrate translocation. Transmembrane and lateral phospholipid asymmetries are not absolute as is the case for integral membrane proteins where asymmetry does not have to be actively maintained due to the enormous energy required to flip across the hydrophobic barrier of the membrane.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |